Processes used to produce transgenic species Question (1 Viewer)

[Celty]

Hi guys,

I just have a question about the dotpoint:
Outline the processes used to produce transgenic species and include examples of this process and reasons for its use.

I have my examples and reasons for use, but I'm not sure how in-depth I need to be when I outline the processes used to produce transgenic species?

I'm not sure if it is sufficient to say they are produced by identifying and removing segments of DNA related to a specific gene, copying it and inserting it into a different species through a gene gun or vectors?

Please help!

 

[superSAIyan2]

This is my example for the bovine-growth hormone salmon. (probably applicable to most transgenic animals)

1. Desirable gene coding for bovine growth hormone is identified on the chromosome and remove using specialised restriction enzymes.
2. Regulatory promoter sequences are added which allow the isolated gene to function and be expressed.
3. Multiple copies of the gene are made using polymerase chain reaction (PCR) technique. Alternatively you can clone using a bacterial plasmid.
4. The gene is inserted into the egg cell of the salmon using microinjection, a gene gun or viral vector.
5. The gene for BGH is expressed in the salmon offspring.

So i guess your process is the same. You might wanna add detail on how the gene is copied and the fact that its expressed in the offspring.